The Titan Krios G3 cryo transmission electron microscope (cryo-TEM) is the most powerful high resolution electron microscope specializing in cryo-based 3D characterization of biological samples. It is equipped with:

• A Schottky X-FEG high brightness electron source.

• Cs image corrector for compensation of spherical aberration (Cs) and semi-automated correction of astigmatism [0.08 nm (TEM) information limit].

• Flexible high tension range from 60 to 300 kV.

• Symmetric constant power C-TWIN objective lens with wide pole piece gap of 11 mm.

• Cryo autoloader for robotic loading of up to 12 frozen, hydrated samples.

• Volta phase plate solution for tunable phase shifting to optimize contrast.

• Low dose software kit to minimize electron dosage.

• Semi-automated data collection for continuous multi-day data acquisition.

• Falcon 3EC pre-GIF direct electron detector camera.

• Selectris energy filter coupled to a Falcon 4i direct electron detector camera..

• Automatic apertures and reproducible recall.

• Rotation-free imaging for clear orientation.

• A computerized 5-axis specimen stage with piezo-enabled drift compensation.