This is a second-generation dedicated cryo-DualBeam focused ion beam microscope for preparing cryo-lamella samples for TEM tomography of frozen-hydrated biological materials. It is equipped with:

• NICol UHR non-immersion field emission-SEM column and Sidewinder ion column.

• In-lens detection system: segmented lower (T1) and upper detector (T2), Everhart-Thornley SE Detector (ETD), ion conversion and electron (ICE) detector for secondary ions and secondary electrons.

• 110 x 110 mm eucentric stage, Fully rotatable cryo-stage (< -170° C at the sample), Tilt range at cryo (eucentric WD): -15° to +55°.

• Automatic Aperture System.

• CCD IR Camera, In-chamber Nav-Cam.

• Cryo Kit, incl. Cryo transfer system, Cryo loading station, conductive deposition GIS, In-chamber retractable sputter coater, EasyLift nanomanipulator, Cryo-FIB AutoGrids.

• Maps 3 software for S/TEM with correlative workflow, AutoScript DB academic, AutoSlice & View, AutoTEM, Cryo for milling automation, SPI™ and iSPI™ software, etc.

• Integrative Fluorescence Light Microscope (iFLM) correlative fluorescence imaging and ion milling