The Monolith MST is used to analyze the interactions between glycomaterials and biomolecules and is based on the detection of a temperature-induced change in fluorescence of a target as a function of the concentration of a non-fluorescent ligand. Unlike surface plasmon resonance (SPR), which immobilizes molecules often causing them to lose activity, the Monolith measures binding interaction in solution. Measuring Kds in solution allows both binding partners to freely interact in their native confirmation.

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